3/4/2023 0 Comments Root primordia(c) The innermost cell of the cortex is the endodermis (En). The Air cavity (Ac) was formed in the cortex. The cortex is consists of parenchyma cells (Pa). Under the epidermis is the exodermis (Ex). (b) The character of the epidermis and cortex of the adventitious root. The root is consists of the epidermis (Ep), cortex (Co), and stele (St). (a) The young adventitious root formed in the stem. Co: cortex St: stele Va: vascular tissue Rc: root cap Sc: stem cell La: lacuna El: elongation region Ma: maturity regionĪnatomic structure of the adventitious root. (i) Developed adventitious root primordium with differentiated root cap (Rc), a region of stem cells (Sc), the region of elongation (El), and maturity region (Ma). Lacuna (La) is formed between the adventitious root primordium and the cortex of the stem. At the outer part of the root tip formed root cap (Rc) surrounded the stem cells. The stem cells (Sc) were protoplasm-rich (dark-stained) and cubical shaped. (g-i) The development of the adventitious root primordium. (f) Cell division and expansion lead to the increased size of the developing adventitious root primordium and forming a dome-like structure (circle). (e) Larger and more advanced adventitious root primordium (circle) with dividing meristematic cells. (d) The dividing cells (arrowhead) in the early stages of adventitious root primordium. (c) Early stages of cell divisions where an adventitious root primordium is initiated (circle). (b) Some of the parenchyma cells outside the vascular tissue circle between cortex and stele carried out the periclinal division (arrowhead). Parenchyma cells surrounded the vascular tissues are large and round. Some of the primary vessels of the vascular bundles were lignified (dark-stained). (a) Vascular tissues on the border between cortex (Co) and stele (St) of the stem. bracteatus adventitious root growth regulator root primordia slip.Īdventitious root primordia formation inside the slip stem of A. The number of primary xylem poles in AR is about 30.Īnanas comosus var. The vascular tissues in the AR develop and are connected with vascular tissue of the stem before the AR grew out the stem. The AR primordia originates from the parenchyma cells located on the borderline between the cortex and stele. The slip stem structure is quite different from other monocots, which consists of epidermis, cortex, and stele with vascular tissues distributed in the cortex and stele. The proper supplementary of ABT can enhance the soluble sugar content, soluble protein content, polyphenol oxidase (PPO) activity and peroxidase (POD) enzyme activity, which resulted in the improvement of rooting. The results showed that, slip cuttings treated with 100 mg/L of Aminobenzotriazole (ABT) for 6 hrs, cultured in river sand: coconut chaff: garden soil 2:2:1 medium is the optimal method for rooting. In order to screen the optimal methods for slips rooting and reveal the anatomic procedure of slip rooting, this study induced slip rooting by different treatment of growth regulator, culture medium, observed the slip stem structure, AR origination and formation procedure through paraffin sections. Growth regulator treatment, and culture medium are imperative factors that affect slip growth and rooting. Adventitious root (AR) induction is essential for the propagation of Ananas comosus var. There are about 4-6 slips on a fruit, and they are good materials for effective regeneration of Ananas comosus var.
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